Survey of polyphenol constituents in grapes and grape-derived products

J Agric Food Chem. 2011 Oct 12;59(19):10586-93. doi: 10.1021/jf202438d. Epub 2011 Sep 13.


A rapid and comprehensive qualitative method has been developed to characterize the different classes of polyphenols, such as anthocyanins, flavonols, phenolic acids, and flavanols/proanthocyanidins, in grape products. The detection was achieved by two runs with the same LC gradient in different MS ionization modes and mobile phase modifiers (positive ionization mode and 0.4% trifluoroacetic acid for anthocyanins and flavonols; negative ionization mode and 0.1% formic acid for phenolic acids and flavanols). From an analysis of the MS and UV data and in comparison with the authenticated standards, a total of 53 compounds were identified, including 33 anthocyanins, 12 flavonols, 4 phenolic acids, and 4 flavanols/proanthocyanidins. With the method developed, a survey was then conducted to qualitatively assess the composition of polyphenols among 29 different grape products including original grape, grape juice, grape wine, and grape-derived dietary supplements, and their chemical profiles were systematically compared. This method provided a comprehensive qualitative insight into the composition of polyphenols in grape-derived products.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anthocyanins / analysis
  • Beverages / analysis
  • Chromatography, High Pressure Liquid
  • Flavonoids / analysis
  • Fruit / chemistry*
  • Hydroxybenzoates / analysis
  • Mass Spectrometry
  • Polyphenols / analysis*
  • Seeds / chemistry
  • Vitis / chemistry*
  • Wine / analysis


  • Anthocyanins
  • Flavonoids
  • Hydroxybenzoates
  • Polyphenols
  • phenolic acid