Molecular and Functional Differences in Voltage-Activated Sodium Currents Between GABA Projection Neurons and Dopamine Neurons in the Substantia Nigra

J Neurophysiol. 2011 Dec;106(6):3019-34. doi: 10.1152/jn.00305.2011. Epub 2011 Aug 31.

Abstract

GABA projection neurons (GABA neurons) in the substantia nigra pars reticulata (SNr) and dopamine projection neurons (DA neurons) in substantia nigra pars compacta (SNc) have strikingly different firing properties. SNc DA neurons fire low-frequency, long-duration spikes, whereas SNr GABA neurons fire high-frequency, short-duration spikes. Since voltage-activated sodium (Na(V)) channels are critical to spike generation, the different firing properties raise the possibility that, compared with DA neurons, Na(V) channels in SNr GABA neurons have higher density, faster kinetics, and less cumulative inactivation. Our quantitative RT-PCR analysis on immunohistochemically identified nigral neurons indicated that mRNAs for pore-forming Na(V)1.1 and Na(V)1.6 subunits and regulatory Na(V)β1 and Na(v)β4 subunits are more abundant in SNr GABA neurons than SNc DA neurons. These α-subunits and β-subunits are key subunits for forming Na(V) channels conducting the transient Na(V) current (I(NaT)), persistent Na current (I(NaP)), and resurgent Na current (I(NaR)). Nucleated patch-clamp recordings showed that I(NaT) had a higher density, a steeper voltage-dependent activation, and a faster deactivation in SNr GABA neurons than in SNc DA neurons. I(NaT) also recovered more quickly from inactivation and had less cumulative inactivation in SNr GABA neurons than in SNc DA neurons. Furthermore, compared with nigral DA neurons, SNr GABA neurons had a larger I(NaR) and I(NaP). Blockade of I(NaP) induced a larger hyperpolarization in SNr GABA neurons than in SNc DA neurons. Taken together, these results indicate that Na(V) channels expressed in fast-spiking SNr GABA neurons and slow-spiking SNc DA neurons are tailored to support their different spiking capabilities.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Animals
  • Animals, Newborn
  • Biophysical Phenomena / drug effects
  • Biophysics
  • Calcium Chloride / pharmacology
  • Cesium / pharmacology
  • Chlorides / pharmacology
  • Dopaminergic Neurons / physiology*
  • Electric Stimulation
  • Female
  • GABAergic Neurons / physiology*
  • Gene Expression
  • Glutamate Decarboxylase / genetics
  • Glutamate Decarboxylase / metabolism
  • In Vitro Techniques
  • Ion Channel Gating / drug effects
  • Ion Channel Gating / genetics
  • Ion Channel Gating / physiology*
  • Male
  • Patch-Clamp Techniques
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Channel Blockers / pharmacology
  • Sodium Channels / classification
  • Sodium Channels / drug effects
  • Sodium Channels / genetics*
  • Sodium Channels / metabolism*
  • Substantia Nigra / cytology*
  • Temperature
  • Tetrodotoxin / pharmacology
  • Tyrosine 3-Monooxygenase / genetics
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Chlorides
  • Sodium Channel Blockers
  • Sodium Channels
  • Cesium
  • Tetrodotoxin
  • Tyrosine 3-Monooxygenase
  • Glutamate Decarboxylase
  • glutamate decarboxylase 1
  • cesium chloride
  • Calcium Chloride