Dihydrotestosterone-inducible IL-6 inhibits elongation of human hair shafts by suppressing matrix cell proliferation and promotes regression of hair follicles in mice

J Invest Dermatol. 2012 Jan;132(1):43-9. doi: 10.1038/jid.2011.274. Epub 2011 Sep 1.

Abstract

Autocrine and paracrine factors are produced by balding dermal papilla (DP) cells following dihydrotestosterone (DHT)-driven alterations and are believed to be key factors involved in male pattern baldness. Herein we report that the IL-6 is upregulated in balding DP cells compared with non-balding DP cells. IL-6 was upregulated 3 hours after 10-100 nM DHT treatment, and ELISA showed that IL-6 was secreted from balding DP cells in response to DHT. IL-6 receptor (IL-6R) and glycoprotein 130 (gp130) were expressed in follicular keratinocytes, including matrix cells. Recombinant human IL-6 (rhIL-6) inhibited hair shaft elongation and suppressed proliferation of matrix cells in cultured human hair follicles. Moreover, rhIL-6 injection into the hypodermis of mice during anagen caused premature onset of catagen. Taken together, our data strongly suggest that DHT-inducible IL-6 inhibits hair growth as a paracrine mediator from the DP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alopecia / metabolism
  • Alopecia / pathology*
  • Alopecia / physiopathology
  • Androgens / pharmacology
  • Animals
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cells, Cultured
  • Cytokine Receptor gp130 / physiology
  • Dermis / cytology
  • Dihydrotestosterone / pharmacology*
  • Female
  • Hair / drug effects
  • Hair / growth & development*
  • Hair Follicle / drug effects*
  • Hair Follicle / pathology*
  • Hair Follicle / physiology
  • Humans
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism*
  • Interleukin-6 / pharmacology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Paracrine Communication / drug effects
  • Paracrine Communication / physiology
  • RNA, Messenger / metabolism
  • Scalp / cytology
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • Androgens
  • IL6 protein, human
  • Interleukin-6
  • RNA, Messenger
  • Dihydrotestosterone
  • Cytokine Receptor gp130