Interleukin-8 derived from local tissue-resident stromal cells promotes tumor cell invasion

Mol Carcinog. 2012 Nov;51(11):861-8. doi: 10.1002/mc.20854. Epub 2011 Aug 31.

Abstract

The aim of this study is to evaluate the role of adipose tissue resident stromal cells on tumor cell invasion. Our data show that a subpopulation of adipose tissue derived stromal cells expressing Nestin, NG2, α-smooth muscle actin and PDGFR-α migrate toward the cancer cells. Microarray analysis revealed the upregulation of IL-8 in the migrated cells. We demonstrated that stromal cell derived IL-8 promote the invasion and the anchorage-independent growth of cancer cells. We conclude that human breast cancer cells attract a subpopulation of stromal cells that secrete IL-8 to promote tumor cell invasion in a paracrine fashion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipose Tissue / cytology*
  • Adipose Tissue / immunology*
  • Adipose Tissue / metabolism
  • Adipose Tissue / pathology
  • Breast Neoplasms / immunology*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Coculture Techniques
  • Female
  • Humans
  • Interleukin-8 / genetics
  • Interleukin-8 / immunology*
  • Neoplasm Invasiveness / immunology*
  • Neoplasm Invasiveness / pathology
  • Oligonucleotide Array Sequence Analysis
  • Stromal Cells / immunology*
  • Stromal Cells / metabolism
  • Stromal Cells / pathology
  • Tumor Microenvironment
  • Up-Regulation

Substances

  • Interleukin-8