Inactivation of herpes simplex virus type 1 & adenovirus type 5 by direct electric current at a biocompatible level in vitro

Clin Lab. 2011;57(7-8):489-95.

Abstract

Background: In vitro studies were conducted to quantify the effectiveness of low-level direct electric current (DC) on infectivity of Herpes Simplex Virus type 1 (HSV-1), Adenovirus type 5 (AdV-5), and on viability of Vero cells.

Methods: Both viruses and Vero cells were exposed to DC by using platinum electrodes connected to a DC source, then the viral infectivity and cell viability were assessed by plaque and MTT assay, respectively.

Results: The results showed that both viruses were inactivated completely by 200 microA DC in 10 minutes (current density = 20 microA/mm2) while this amount of DC had no significant changes on the viability of Vero cells (viability > 90 %). Inactivation degree of HSV-1 and AdV-5 was 5 and 4 log per mL, respectively. Further study is required to investigate the mechanism of inactivation by this method.

Conclusions: DC at a biocompatible level showed the competency to inactivate the viruses in the solution completely. So it is a useful tool for designing a noninvasive method for decontamination of biological or synthetic fluids. This method or derivation can be considered as an easy, fast, and economical method for fluid decontamination.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adenoviruses, Human* / growth & development
  • Animals
  • Cell Survival
  • Chlorocebus aethiops
  • Decontamination / methods*
  • Electricity*
  • Equipment Design
  • Herpesvirus 1, Human* / growth & development
  • In Vitro Techniques
  • Vero Cells / cytology
  • Vero Cells / virology
  • Viral Plaque Assay
  • Virus Inactivation*