Prospective study on the incidence of infection by Borrelia burgdorferi sensu lato after a tick bite in a highly endemic area of Switzerland

Ticks Tick Borne Dis. 2011 Sep;2(3):129-36. doi: 10.1016/j.ttbdis.2011.05.002. Epub 2011 Jul 28.

Abstract

The periurban forest of Neuchâtel (Switzerland) is a high-risk area for Lyme Borreliosis, due to a high density of infected Ixodes ricinus ticks. In this study, we evaluated the risk of subclinical (seroconversion) and clinical infection after a tick bite in Neuchâtel inhabitants from 2003 to 2005. Inhabitants have been invited, through media, to visit a physician after a tick bite. A questionnaire was filled out and two blood samples were taken at 8-week interval. EIA screening tests for IgM and IgG (IMX system, Abbott) were applied for paired sera. In case of a change in antibody titres between both samples, a homemade Western-blot using Borrelia afzelii, B. burgdorferi sensu stricto and B. garinii as antigens was performed. Participants were included into two groups. Group one included asymptomatic participants (n=255). Among them, nine (3.5%) seroconverted with seroconversion rates varying between 6.8% in 2003, 2.1% in 2004 and 2.3% in 2005. Participants who developed clinical symptoms of LB were included into group two (n=14). Erythema migrans (EM) was reported in 5.2% of participants (5.2%), varying between 7.5% in 2003, 5% in 2004 and 3.4% in 2005. Ticks obtained from 186 participants were examined for B. burgdorferi infection by PCR/Reverse Line Blotting, and by Real Time PCR and tick attachment duration was estimated. Among I. ricinus ticks collected from participants, 32.8% were infected by B. burgdorferi sensu lato. B. afzelii predominated among these ticks. Globally, 65.9% of nymphs remained attached for more than 24h whereas only 38.3% of female ticks remained attached for more than 24h. We observed that 6.6% and 2.4% of participants bitten by infected and uninfected ticks, respectively, developed EM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Arachnid Vectors / microbiology*
  • Arachnid Vectors / physiology
  • Bites and Stings / immunology
  • Bites and Stings / microbiology
  • Blotting, Western
  • Borrelia burgdorferi / isolation & purification*
  • Borrelia burgdorferi Group / isolation & purification*
  • Child
  • Child, Preschool
  • DNA, Bacterial / analysis
  • Female
  • Glossitis, Benign Migratory / blood
  • Glossitis, Benign Migratory / diagnosis
  • Glossitis, Benign Migratory / epidemiology*
  • Glossitis, Benign Migratory / microbiology
  • Humans
  • Immunoglobulin G / analysis
  • Immunoglobulin G / immunology
  • Immunoglobulin M / analysis
  • Immunoglobulin M / immunology
  • Incidence
  • Infant
  • Ixodes / microbiology*
  • Ixodes / physiology
  • Lyme Disease / blood
  • Lyme Disease / diagnosis
  • Lyme Disease / epidemiology*
  • Lyme Disease / immunology
  • Lyme Disease / microbiology
  • Lyme Disease / transmission
  • Male
  • Middle Aged
  • Nymph / microbiology*
  • Nymph / physiology
  • Prospective Studies
  • Real-Time Polymerase Chain Reaction
  • Serologic Tests
  • Switzerland / epidemiology

Substances

  • DNA, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M