Identification and characterisation of the egr-1 gene product as an inducible, short-lived, nuclear phosphoprotein

Oncogene. 1990 May;5(5):669-74.


The egr-1 gene (also termed NGFI-A, zif/268 and Krox24) is predicted to encode a protein with three 'zinc finger' domains. Analogous DNA-binding motifs are present in several putative transcriptional regulatory proteins. The mRNA of egr-1 is rapidly and transiently induced by a variety of growth factors in diverse cell types. We have used a synthetic peptide corresponding to the C-terminus of the predicted egr-1 protein to generate mouse and rabbit polyclonal antibodies. These antibodies have been used to identify the egr-1 protein, designated p75egr-1, by immunoprecipitation, immunoblotting and immunocytochemical analyses. p75egr-1 is induced within 1-2 h of mitogenic stimulation and has a half-life of under 2 h. The protein is phosphorylated and has been localised to the nucleus by both immunocytochemical analysis and biochemical fractionation. The egr-1 protein is released from nuclei under isotonic or hypertonic salt conditions or by treatment with DNAase and binds double-stranded DNA in vitro. Consistent with the distribution described for the mRNA the egr-1 protein is abundantly expressed in normal rat brain.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Brain / cytology
  • Brain / metabolism
  • Cell Nucleus / metabolism
  • DNA / genetics
  • DNA / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / immunology
  • DNA-Binding Proteins / metabolism
  • Early Growth Response Protein 1
  • Fluorescent Antibody Technique
  • Immediate-Early Proteins*
  • Mice
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / immunology
  • Nuclear Proteins / metabolism
  • Phosphoproteins / genetics*
  • Phosphoproteins / immunology
  • Phosphoproteins / metabolism
  • Precipitin Tests
  • Rats
  • Transcription Factors / genetics*
  • Transcription Factors / immunology
  • Transcription Factors / metabolism


  • Antibodies
  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, mouse
  • Egr1 protein, rat
  • Immediate-Early Proteins
  • Nuclear Proteins
  • Phosphoproteins
  • Transcription Factors
  • DNA