FUdR causes a twofold increase in the lifespan of the mitochondrial mutant gas-1

Mech Ageing Dev. 2011 Oct;132(10):519-21. doi: 10.1016/j.mad.2011.08.006. Epub 2011 Aug 27.


The nematode worm Caenorhabditis elegans has been used to identify hundreds of genes that influence longevity and thereby demonstrate the strong influence of genetics on lifespan determination. In order to simplify lifespan studies in worms, many researchers have employed 5-fluoro-2'-deoxyuridine (FUdR) to inhibit the development of progeny. While FUdR has little impact on the lifespan of wild-type worms, we demonstrate that FUdR causes a dramatic, dose-dependent, twofold increase in the lifespan of the mitochondrial mutant gas-1. Thus, the concentration of FUdR employed in a lifespan study can determine whether a particular strain is long-lived or short-lived compared to wild-type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins / genetics*
  • Dose-Response Relationship, Drug
  • Floxuridine / administration & dosage
  • Floxuridine / pharmacology*
  • Genes, Helminth*
  • Genes, Mitochondrial*
  • Longevity / drug effects*
  • Longevity / genetics*
  • Mutation
  • NADH Dehydrogenase / genetics*


  • Caenorhabditis elegans Proteins
  • Floxuridine
  • NADH Dehydrogenase
  • GAS-1 protein, C elegans