Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples

Mem Inst Oswaldo Cruz. 2011 Aug;106(5):613-6. doi: 10.1590/s0074-02762011000500014.

Abstract

In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil / epidemiology
  • Genetic Variation*
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics*
  • Humans
  • Influenza A Virus, H1N1 Subtype / genetics*
  • Influenza, Human / mortality
  • Influenza, Human / virology*
  • Molecular Sequence Data
  • Mutation / genetics*
  • Pandemics*
  • Phylogeny
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis

Substances

  • H1N1 virus hemagglutinin
  • Hemagglutinin Glycoproteins, Influenza Virus
  • RNA, Viral