The FUR1 gene of Saccharomyces cerevisiae: cloning, structure and expression of wild-type and mutant alleles

Gene. 1990 Apr 16;88(2):149-57. doi: 10.1016/0378-1119(90)90026-n.


The FUR1 gene of Saccharomyces cerevisiae encodes uracil phosphoribosyltransferase (UPRTase) which catalyses the conversion of uracil into uridine 5'-monophosphate (UMP) in the pyrimidine salvage pathway. The FUR1 gene is included in a 2.1 kb genomic segment of DNA and is transcribed into a 1 kb poly(A)+mRNA. Sequencing has determined a 753 bp open reading frame capable of encoding a protein of 251 amino acids. The FUR1 genes for three recessive fur1 alleles, having different sensibilities to 5-fluorouridine (5-FUR) but identical levels of resistance to 5-fluorouracil (5-FU), were cloned and sequenced. Single bp changes located in different regions of the gene were found in each mutant. Two in vitro-constructed deletions of the FUR1 gene have been integrated at the chromosomal locus, giving strains with 5-FURR and 5-FURR mutant phenotype. Assays of UPRTase, uridine kinase, uridine ribohydrolase and uridine 5'-monophosphate nucleotidase enzymatic activities, in extracts of strains where the FUR1 gene is overexpressed or deleted, indicate that the FUR1 encoded protein possesses only UPRTase activity.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA Mutational Analysis
  • DNA, Recombinant
  • Fluorouracil / metabolism
  • Genes, Fungal / genetics*
  • Molecular Sequence Data
  • Orotate Phosphoribosyltransferase / genetics
  • Pentosyltransferases / genetics*
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Uracil / metabolism
  • Uridine Monophosphate / metabolism


  • DNA, Recombinant
  • Uracil
  • Uridine Monophosphate
  • Pentosyltransferases
  • Orotate Phosphoribosyltransferase
  • uracil phosphoribosyltransferase
  • Fluorouracil

Associated data

  • GENBANK/M36485