Spectral characteristics of diphenylamine (DPA) have been investigated in β-cyclodextrin (β-CDx) solution. The formation of the complex was revealed by UV, steady state and time-resolved fluorescence spectroscopy. The stoichiometry of DPA:β-CDx complex, determined using Benesi-Hildebrand equation and Job's continuous variation method is 1:1. The binding constants calculated from various methods are reported. This inclusion complex formation from DPA and β-CDx was also confirmed by the FT-IR spectral study and SEM image analysis of solid complex prepared by co-precipitation method.
Copyright © 2011 Elsevier B.V. All rights reserved.