Assessment of antibody specificity using suspension bead arrays

Methods Mol Biol. 2011;785:183-9. doi: 10.1007/978-1-61779-286-1_12.

Abstract

With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies* / metabolism
  • Antibody Specificity
  • Antigens / metabolism*
  • Microspheres*
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods*

Substances

  • Antibodies
  • Antigens