Protection of mice against tumor growth by immunization with an oncogene-encoded growth factor

Proc Natl Acad Sci U S A. 1990 Jun;87(11):4222-5. doi: 10.1073/pnas.87.11.4222.


The K-fgf/hst oncogene encodes a growth factor of the fibroblast growth factor (FGF) family that is secreted and transforms cells through a mechanism of autocrine cell proliferation. K-fgf-transformed cells are highly tumorigenic in immunocompetent allogeneic and syngeneic animals. BALB/c mice were immunized with a bacterial fusion protein consisting of a portion of the MS2 polymerase and of the human K-FGF precursor lacking only the first 4 amino acids or with a recombinant protein corresponding to the mature, secreted form of K-FGF (176 amino acids). They were then challenged with syngeneic K-fgf- or H-ras-transformed cells. Vaccinated animals exhibited a significant degree of protection against tumor induction, which was specific for K-fgf-transformed cells and correlated with the ability of the immunized mice to produce high titers of anti-K-FGF antibodies. Thus immunization with a single oncogene product can protect animals against tumor cells expressing this oncogene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Neoplasm / immunology
  • Cell Transformation, Neoplastic
  • Enzyme-Linked Immunosorbent Assay
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors / genetics
  • Fibroblast Growth Factors / immunology*
  • Immunotherapy
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Neoplasms, Experimental / pathology*
  • Neoplasms, Experimental / therapy
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / immunology*
  • Recombinant Fusion Proteins


  • Antibodies, Neoplasm
  • Fgf4 protein, mouse
  • Fibroblast Growth Factor 4
  • Proto-Oncogene Proteins
  • Recombinant Fusion Proteins
  • Fibroblast Growth Factors