Solution NMR structure of VF0530 from Vibrio fischeri reveals a nucleic acid-binding function

Proteins. 2011 Oct;79(10):2988-91. doi: 10.1002/prot.23121. Epub 2011 Aug 26.


Protein domain family PF09905 (DUF2132) is a family of small domains of unknown function that are conserved in a wide range of bacteria. Here we describe the solution NMR structure of the 80-residue VF0530 protein from Vibrio fischeri, the first structural representative from this protein domain family. We demonstrate that the structure of VF0530 adopts a unique four-helix motif that shows some similarity to the C-terminal double-stranded DNA (dsDNA) binding domain of RecA, as well as other nucleic acid binding domains. Moreover, gel shift binding data indicate a potential dsDNA binding role for VF0530.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aliivibrio fischeri / metabolism*
  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Magnetic Resonance Spectroscopy / methods*
  • Nucleic Acids / chemistry*
  • Nucleic Acids / metabolism*
  • Protein Binding
  • Protein Structure, Secondary


  • Bacterial Proteins
  • Nucleic Acids