Induction of haptotactic migration of melanoma cells by neutrophil activating protein/interleukin-8

Biochem Biophys Res Commun. 1990 May 31;169(1):165-70. doi: 10.1016/0006-291x(90)91449-3.


Natural or recombinant neutrophil activating cytokine (IL-8) induced migration across polycarbonate filters of human A 2058 melanoma cells. Anti-IL-8 antibodies blocked IL-8 induced melanoma cell migration. Checkerboard experiments revealed a gradient-dependent response of A2058 melanoma cells to IL-8. Filters exposed to IL-8 and washed supported melanoma cell migration, thus implying a haptotactic component in the response. The homologous polypeptide platelet factor 4 was inactive. The observation that IL-8 affects melanoma cells emphasizes the need for a comprehensive analysis of the spectrum of action of platelet factor 4-related peptides. The effect of the inflammatory cytokine IL-8 on melanoma cells may be relevant to augmented secondary localization of tumors at sites of inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemotactic Factors / pharmacology*
  • Chemotaxis / drug effects*
  • Humans
  • Interleukin-8
  • Interleukins / pharmacology*
  • Melanoma / pathology*
  • Platelet Factor 4 / pharmacology
  • Recombinant Proteins / pharmacology
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / pathology


  • Chemotactic Factors
  • Interleukin-8
  • Interleukins
  • Recombinant Proteins
  • Platelet Factor 4