The effects of gold nanoparticles in wound healing with antioxidant epigallocatechin gallate and α-lipoic acid

Nanomedicine. 2012 Jul;8(5):767-75. doi: 10.1016/j.nano.2011.08.013. Epub 2011 Sep 9.


Topical applications of antioxidant agents in cutaneous wounds have attracted much attention. Gold nanoparticles (AuNPs), epigallocatechin gallate (EGCG), and α-lipoic acid (ALA) were shown to have antioxidative effects and could be helpful in wound healing. Their effects in Hs68 and HaCaT cell proliferation and in mouse cutaneous wound healing were studied. Both the mixture of EGCG + ALA (EA) and AuNPs + EGCG + ALA (AuEA) significantly increased Hs68 and HaCaT proliferation and migration. Topical AuEA application accelerated wound healing on mouse skin. Immunoblotting of wound tissue showed significant increase of vascular endothelial cell growth factor and angiopoietin-1 protein expression, but no change of angiopoietin-2 or CD31 after 7 days. After AuEA treatment, CD68 protein expression decreased and Cu/Zn superoxide dismutase increased significantly in the wound area. In conclusion, AuEA significantly accelerated mouse cutaneous wound healing through anti-inflammatory and antioxidation effects. This study may support future studies using other antioxidant agents in the treatment of cutaneous wounds.

From the clinical editor: In this study, topically applied gold nanoparticles with epigallocatechin gallate and alpha-lipoic acid were studied regarding their effects in wound healing in cell cultures. Significant acceleration was demonstrated in wound healing in a murine model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage*
  • Catechin / administration & dosage
  • Catechin / analogs & derivatives*
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Gene Expression / drug effects
  • Gold / administration & dosage*
  • Gold / chemistry
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Neovascularization, Physiologic
  • Ribonuclease, Pancreatic / metabolism
  • Skin / drug effects
  • Skin / injuries
  • Thioctic Acid / administration & dosage*
  • Vascular Endothelial Growth Factor A / metabolism
  • Wound Healing / drug effects


  • Antioxidants
  • Vascular Endothelial Growth Factor A
  • vascular endothelial growth factor A, mouse
  • Thioctic Acid
  • Gold
  • Catechin
  • epigallocatechin gallate
  • angiogenin
  • Ribonuclease, Pancreatic