Peptide immunoaffinity enrichment coupled with mass spectrometry for peptide and protein quantification

Clin Lab Med. 2011 Sep;31(3):385-96. doi: 10.1016/j.cll.2011.07.004.

Abstract

Quantitative protein assays are needed in a wide range of biological studies. Traditional immunoassays are not available for a large number of proteins and development of new immunoassays requires a significant investment in time and money. The development of assays using peptide immunoaffinity enrichment coupled with targeted mass spectrometry has many advantages including versatility in design, ease of use, enhanced specificity, and good performance characteristics. This review presents recent developments in the characterization and implementation of immuno-SRM assays.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Antibodies / immunology
  • Humans
  • Immunoassay*
  • Isotope Labeling / methods
  • Limit of Detection
  • Mass Spectrometry / methods*
  • Peptides / analysis
  • Peptides / immunology
  • Plasma / chemistry
  • Proteins / analysis*
  • Proteins / immunology
  • Proteomics / methods*
  • Trypsin / metabolism

Substances

  • Antibodies
  • Peptides
  • Proteins
  • Trypsin