DNA demethylation in PD-1 gene promoter induced by 5-azacytidine activates PD-1 expression on Molt-4 cells

Cell Immunol. 2011;271(2):450-4. doi: 10.1016/j.cellimm.2011.08.014. Epub 2011 Aug 23.


The expression of the programmed death 1 (PD-1) gene is an indicator of exhausted T-cells with decreased activation and function. It remains unknown, however, whether the methylation status of the PD-1 gene promoter is associated with PD-1 expression level. This study shows the changes of PD-1 expression levels and the demethylation status of the PD-1 promoter region in Molt-4 cells under different concentrations of 5-azacytidine (5-Zac). The result demonstrated that DNA demethylation at PD-1 promoter may contribute to PD-1 overexpression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Azacitidine / pharmacology*
  • Base Sequence
  • Cell Line, Tumor
  • CpG Islands
  • DNA Methylation / drug effects
  • DNA Primers / genetics
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / metabolism
  • Gene Expression / drug effects
  • Humans
  • Programmed Cell Death 1 Receptor / genetics*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism


  • DNA Primers
  • DNA, Neoplasm
  • PDCD1 protein, human
  • Programmed Cell Death 1 Receptor
  • RNA, Messenger
  • RNA, Neoplasm
  • Azacitidine