β-Carotene and lutein inhibit hydrogen peroxide-induced activation of NF-κB and IL-8 expression in gastric epithelial AGS cells

J Nutr Sci Vitaminol (Tokyo). 2011;57(3):216-23. doi: 10.3177/jnsv.57.216.

Abstract

Reactive oxygen species (ROS) including hydrogen peroxide (H(2)O(2)) are involved in the pathogenesis of gastric inflammation. Interleukin-8 (IL-8) is a potent mediator of the inflammatory response by activating and recruiting neutrophils to the site of infection. Oxidant-sensitive transcription factor NF-κB regulates the expression of IL-8 in the immune and inflammatory events. Carotenoids (carotenes and oxygenated carotenoids) show antioxidant and anti-inflammatory activities. Low intake of β-carotene leads to high risk of gastric cancer. Oxygenated carotenoid lutein inhibited NF-κB activation in experimental uveitis. The present study aims to investigate whether β-carotene and lutein inhibit H(2)O(2)-induced activation of NF-κB and expression of IL-8 in gastric epithelial AGS cells. The cells were treated with carotenoids 2 h prior to the treatment of H(2)O(2). mRNA expression was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and real time RT-PCR analyses. IL-8 level in the medium was determined by enzyme-linked immunosorbent assay. NF-κB activation was assessed by electrophoretic mobility shift assay. ROS levels of the cells were detected by confocal microscopic analysis for fluorescent dichlorofluorescein. As a result, H(2)O(2 )induced the activation of NF-κB and expression of IL-8 in AGS cells time-dependently. β-Carotene and lutein showed inhibitory effects on H(2)O(2)-induced increase in intracellular ROS levels, activation of NF-κB, and IL-8 expression in AGS cells. In conclusion, supplementation of carotenoids such as β-carotene and lutein may be beneficial for the treatment of oxidative stress-mediated gastric inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / pharmacology*
  • Cell Line, Tumor
  • Epithelial Cells / drug effects*
  • Epithelial Cells / metabolism
  • Gastric Mucosa / metabolism
  • Humans
  • Hydrogen Peroxide
  • Inflammation / drug therapy
  • Inflammation Mediators / antagonists & inhibitors
  • Interleukin-8 / metabolism*
  • Lutein / pharmacology*
  • NF-kappa B / metabolism*
  • RNA, Messenger / metabolism
  • Reactive Oxygen Species / metabolism
  • Stomach / drug effects
  • beta Carotene / pharmacology*

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Inflammation Mediators
  • Interleukin-8
  • NF-kappa B
  • RNA, Messenger
  • Reactive Oxygen Species
  • beta Carotene
  • Hydrogen Peroxide
  • Lutein