DNA reorientation on Au nanoparticles: label-free detection of hybridization by surface enhanced Raman spectroscopy

Chem Commun (Camb). 2011 Oct 21;47(39):10966-8. doi: 10.1039/c1cc13705k. Epub 2011 Sep 12.

Abstract

DNA sequences attached to Au nanoparticles via thiol linkers stand up from the surface, giving preferential enhancement of the adenine ring breathing SERS band. Non-specific binding via the nucleobases reorients the DNA, reducing this effect. This change in intensity on reorientation was utilised for label-free detection of hybridization of a molecular beacon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / genetics
  • Gold / chemistry*
  • Metal Nanoparticles / chemistry*
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / genetics
  • Spectrum Analysis, Raman*
  • Surface Properties

Substances

  • Oligonucleotide Probes
  • Gold
  • DNA