Clinical application of a dendritic cell vaccine raised against heat-shocked glioblastoma

Cell Biochem Biophys. 2012 Jan;62(1):91-9. doi: 10.1007/s12013-011-9265-6.

Abstract

Establishment of a detection platform for glioblastoma-dendritic cell (DC) vaccine preparation and to determine the efficacy of the vaccine in a clinical trial. Autologous glioblastoma-DC vaccine was prepared from a glioblast specimen procured from surgical resection. The specimen was used to enrich the vaccine with peripherally blood-derived DCs after heat-shock induced, glioblastoma apoptosis. The control group received conventional treatment of surgery and radio-chemotherapy post-operation. The therapeutic group received a combination of glioblastoma-DC vaccine and conventional therapy. A comparison of the functional immune parameters, including tumor control, rate live time, Karnofsky scores, and complications occurring in each group were observed and recorded. The proportions of peripheral CD3(+), CD3(+)CD4(+), CD4(+)/CD8(+), and NK cells were significantly higher after DC vaccination than the control group (P < 0.05). Serum levels of IL-2, IL-12, and IFN-γ were significantly higher after DC vaccination than in the control group (P < 0.05). Nine months after vaccination, tumor control rate is significantly improved in the DC group compared with the control group (P < 0.05); survival rate was significantly higher in DC group than in control group (P < 0.05) and the time to relapse was significantly longer in DC group than that in control group (P < 0.05). Karnofsky scores were better in DC vaccination group 6 and 9 months post-treatment compared with the control group (P < 0.05). The combination of glioma DC vaccine and radiotherapy/chemotherapy post-operatively enhances the immune function of patients, increases the tumor control rate, prolongs the survival time and relapse duration, improves the quality of life, and therefore provides a more effective intervention of treating glioblastoma.

Publication types

  • Controlled Clinical Trial

MeSH terms

  • Adult
  • Cancer Vaccines / immunology
  • Cancer Vaccines / therapeutic use*
  • Central Nervous System Neoplasms / drug therapy
  • Central Nervous System Neoplasms / radiotherapy
  • Central Nervous System Neoplasms / therapy*
  • Combined Modality Therapy
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Female
  • Glioblastoma / drug therapy
  • Glioblastoma / radiotherapy
  • Glioblastoma / therapy*
  • Humans
  • Immunotherapy
  • Interferon-gamma / blood
  • Interleukin-12 / blood
  • Interleukin-2 / blood
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / immunology
  • Magnetic Resonance Imaging
  • Male
  • Middle Aged
  • Quality of Life
  • Survival Analysis
  • Temperature

Substances

  • Cancer Vaccines
  • Interleukin-2
  • Interleukin-12
  • Interferon-gamma