NHR-23 dependent collagen and hedgehog-related genes required for molting

Biochem Biophys Res Commun. 2011 Oct 7;413(4):515-20. doi: 10.1016/j.bbrc.2011.08.124. Epub 2011 Sep 2.


NHR-23, a conserved member of the nuclear receptor family of transcription factors, is required for normal development in Caenorhabditis elegans where it plays a critical role in growth and molting. In a search for NHR-23 dependent genes, we performed whole genome comparative expression microarrays on both control and nhr-23 inhibited synchronized larvae. Genes that decreased in response to nhr-23 RNAi included several collagen genes. Unexpectedly, several hedgehog-related genes were also down-regulated after nhr-23 RNAi. A homozygous nhr-23 deletion allele was used to confirm the RNAi knockdown phenotypes and the changes in gene expression. Our results indicate that NHR-23 is a critical co-regulator of functionally linked genes involved in growth and molting and reveal evolutionary parallels among the ecdysozoa.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / growth & development*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Gene Deletion
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins / metabolism*
  • Molecular Sequence Data
  • Molting / genetics*
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*


  • Caenorhabditis elegans Proteins
  • Hedgehog Proteins
  • NHR-23 protein, C elegans
  • Receptors, Cytoplasmic and Nuclear
  • Transcription Factors