Neural differentiation of induced pluripotent stem cells

Methods Mol Biol. 2011;793:99-110. doi: 10.1007/978-1-61779-328-8_7.


The great potential of induced pluripotent cells (iPS) cells is that it allows the possibility of deriving pluripotent stem cells from any human patient. Generation of patient-derived stem cells serves as a great source for developing cell replacement therapies and also for creating human cellular model systems of specific diseases or disorders. This is only of benefit if there are well-established differentiation assay systems to generate the cell types of interest. This chapter describes robust and well-characterized protocols for differentiating iPS cells to neural progenitors, neurons, glia and neural crest cells. These established assays can be applied to iPS cell lines derived from patients with neurodegenerative disorders to study cellular mechanisms associated with neurodegeneration as well as investigating the regenerative potential of patient derived stem cells.

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Carrier Proteins / pharmacology
  • Cell Differentiation* / drug effects
  • Cell Line
  • Coculture Techniques
  • Cytological Techniques / methods*
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / drug effects
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Gene Expression Regulation / drug effects
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects
  • Mice
  • Mitomycin / pharmacology
  • Neural Crest / cytology*
  • Neural Crest / drug effects
  • Neural Crest / metabolism
  • Neurodegenerative Diseases / pathology
  • Neuroglia / cytology*
  • Neuroglia / drug effects
  • Neuroglia / metabolism
  • Neurons / cytology*
  • Neurons / drug effects
  • Neurons / metabolism


  • Biomarkers
  • Carrier Proteins
  • noggin protein
  • Mitomycin