Lower weight gain and hepatic lipid content in hamsters fed high fat diets supplemented with white rice protein, brown rice protein, soy protein, and their hydrolysates

J Agric Food Chem. 2011 Oct 26;59(20):10927-33. doi: 10.1021/jf202721z. Epub 2011 Sep 30.


The physiological effects of the hydrolysates of white rice protein (WRP), brown rice protein (BRP), and soy protein (SP) hydrolyzed by the food grade enzyme, alcalase2.4 L, were compared to the original protein source. Male Syrian Golden hamsters were fed high-fat diets containing either 20% casein (control) or 20% extracted proteins or their hydrolysates as the protein source for 3 weeks. The brown rice protein hydrolysate (BRPH) diet group reduced weight gain 76% compared with the control. Animals fed the BRPH supplemented diet also had lower final body weight, liver weight, very low density lipoprotein cholesterol (VLDL-C), and liver cholesterol, and higher fecal fat and bile acid excretion than the control. Expression levels of hepatic genes for lipid oxidation, PPARα, ACOX1, and CPT1, were highest for hamsters fed the BRPH supplemented diet. Expression of CYP7A1, the gene regulating bile acid synthesis, was higher in all test groups. Expression of CYP51, a gene coding for an enzyme involved in cholesterol synthesis, was highest in the BRPH diet group. The results suggest that BRPH includes unique peptides that reduce weight gain and hepatic cholesterol synthesis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cholesterol / analysis
  • Cholesterol / biosynthesis
  • Cholesterol / blood
  • Cricetinae
  • Dietary Fats / administration & dosage*
  • Dietary Supplements
  • Feces / chemistry
  • Gene Expression
  • Lipid Peroxidation / genetics
  • Lipids / analysis
  • Lipids / blood
  • Liver / anatomy & histology
  • Liver / chemistry*
  • Liver / metabolism
  • Male
  • Mesocricetus
  • Organ Size / drug effects
  • Oryza / chemistry
  • Plant Proteins / administration & dosage*
  • Protein Hydrolysates / administration & dosage*
  • Seeds / chemistry
  • Soybean Proteins / administration & dosage
  • Weight Gain / drug effects*


  • Dietary Fats
  • Lipids
  • Plant Proteins
  • Protein Hydrolysates
  • Soybean Proteins
  • Cholesterol