ICSH recommendations for the measurement of haemoglobin F

Int J Lab Hematol. 2012 Feb;34(1):14-20. doi: 10.1111/j.1751-553X.2011.01367.x. Epub 2011 Sep 14.


Measurement of the Haemoglobin F in red cell haemolysates is important in the diagnosis of δβ thalassaemia, hereditary persistence of fetal haemoglobin (HPFH) and in the diagnosis and management of sickle cell disease. The distribution of Hb F in red cells is useful in the diagnosis of HPFH and in the assessment of feto-maternal haemorrhage. The methods of quantifying Hb F are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high-performance liquid chromatography and capillary electrophoresis.

Publication types

  • Practice Guideline

MeSH terms

  • Alkalies
  • Chromatography, High Pressure Liquid / methods
  • Electrophoresis, Capillary / methods
  • Fetal Hemoglobin / analysis*
  • Flow Cytometry / methods
  • Humans
  • Immunodiffusion / methods
  • Protein Denaturation
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Thalassemia / diagnosis*


  • Alkalies
  • Fetal Hemoglobin