GMC Oxidoreductase, a Highly Expressed Protein in a Potent Biocontrol Agent Fusarium Oxysporum Cong:1-2, Is Dispensable for Biocontrol Activity

J Gen Appl Microbiol. 2011;57(4):207-17. doi: 10.2323/jgam.57.207.

Abstract

A spontaneous non-pathogenic variant (Cong:1-2) derived from Fusarium oxysporum f. sp. conglutinans (Cong: 1-1), a causal agent of cabbage yellows, carries biocontrol activity for cabbage yellows. We found a GMC oxidoreductase (ODX1) among the proteins expressed much more in Cong:1-2 than Cong:1-1 by 2D-DIGE comparison. GMC oxidoreductases have been reported to be involved in biocontrol activity of several plant pathogenic fungi. The gene encoding ODX1 in Cong:1-2 was cloned, and targeted disruption of the gene in Cong:1-2 did not affect its biocontrol activity, suggesting that GMC oxidoreductase is dispensable for biocontrol activity in the fungal biocontrol agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica / microbiology*
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics
  • Fusarium / enzymology*
  • Fusarium / genetics
  • Fusarium / pathogenicity
  • Mutation
  • Oxidoreductases / genetics*
  • Oxidoreductases / metabolism
  • Pest Control, Biological*
  • Phylogeny
  • Plant Diseases / microbiology*
  • Plant Roots / microbiology
  • Seedlings / microbiology
  • Two-Dimensional Difference Gel Electrophoresis
  • Virulence

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Oxidoreductases