New 30-valent M protein-based vaccine evokes cross-opsonic antibodies against non-vaccine serotypes of group A streptococci

Vaccine. 2011 Oct 26;29(46):8175-8. doi: 10.1016/j.vaccine.2011.09.005. Epub 2011 Sep 13.


Our previous studies have shown that recombinant multivalent vaccines containing amino-terminal M protein fragments from as many as 26 different serotypes of group A streptococci (GAS) evoked opsonic antibodies in animals and humans. In the present study, we constructed a new 30-valent vaccine containing M protein peptides from GAS serotypes prevalent in North America and Europe. The vaccine was immunogenic in rabbits and evoked bactericidal antibodies against all 30 vaccine serotypes of GAS. In addition, the vaccine antisera also contained significant levels of bactericidal antibodies against 24 of 40 non-vaccine serotypes of GAS. These results indicate that the potential efficacy of the new multivalent vaccine may be greater than predicted based on the "type-specific" M peptides represented.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / immunology*
  • Blood Bactericidal Activity
  • Carrier Proteins / genetics
  • Carrier Proteins / immunology*
  • Cross Reactions
  • Opsonin Proteins / blood*
  • Rabbits
  • Streptococcal Vaccines / administration & dosage
  • Streptococcal Vaccines / immunology*
  • Streptococcus pyogenes / immunology*
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Carrier Proteins
  • Opsonin Proteins
  • Streptococcal Vaccines
  • Vaccines, Synthetic
  • streptococcal M protein