A genetic in vivo system to detect asymmetrically distributed RNA

EMBO Rep. 2011 Oct 28;12(11):1167-74. doi: 10.1038/embor.2011.178.

Abstract

Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Drosophila melanogaster / genetics*
  • Genetic Testing*
  • Genetic Vectors / genetics
  • Green Fluorescent Proteins / metabolism
  • Levivirus / metabolism
  • Organ Specificity / genetics
  • Pilot Projects
  • Protein Biosynthesis / genetics
  • RNA / metabolism*
  • RNA Transport / genetics*
  • Reproducibility of Results

Substances

  • Green Fluorescent Proteins
  • RNA