Engineering zinc finger nucleases for targeted mutagenesis of zebrafish

Methods Cell Biol. 2011;104:51-8. doi: 10.1016/B978-0-12-374814-0.00003-3.

Abstract

Zinc finger nucleases provide an important platform for performing reverse genetics in zebrafish. Here we review various methods and resources that have been used to create customized zinc finger nucleases for use in zebrafish. We also provide a framework for choosing among the various publicly available platforms available to engineer ZFNs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Cloning, Molecular
  • Decision Trees
  • Deoxyribonucleases, Type II Site-Specific / genetics*
  • Genetic Vectors
  • Mutagenesis, Site-Directed / methods
  • Protein Engineering / methods*
  • Reverse Genetics / methods*
  • Zebrafish / genetics*
  • Zinc Fingers

Substances

  • endodeoxyribonuclease FokI
  • Deoxyribonucleases, Type II Site-Specific