Transgenic zebrafish using transposable elements

Methods Cell Biol. 2011;104:137-49. doi: 10.1016/B978-0-12-374814-0.00008-2.

Abstract

DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Genetically Modified*
  • Cloning, Molecular / methods
  • DNA Transposable Elements*
  • Genetic Testing / methods
  • Genetic Vectors
  • Germ-Line Mutation
  • Microinjections / methods
  • Mutagenesis, Insertional / methods*
  • Zebrafish / genetics*

Substances

  • DNA Transposable Elements