Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection

Cell Host Microbe. 2011 Sep 15;10(3):210-23. doi: 10.1016/j.chom.2011.08.010.

Abstract

Many viruses deliver their genomes into the host cell nucleus for replication. However, the size restrictions of the nuclear pore complex (NPC), which regulates the passage of proteins, nucleic acids, and solutes through the nuclear envelope, require virus capsid uncoating before viral DNA can access the nucleus. We report a microtubule motor kinesin-1-mediated and NPC-supported mechanism of adenovirus uncoating. The capsid binds to the NPC filament protein Nup214 and kinesin-1 light-chain Klc1/2. The nucleoporin Nup358, which is bound to Nup214/Nup88, interacts with the kinesin-1 heavy-chain Kif5c to indirectly link the capsid to the kinesin motor. Kinesin-1 disrupts capsids docked at Nup214, which compromises the NPC and dislocates nucleoporins and capsid fragments into the cytoplasm. NPC disruption increases nuclear envelope permeability as indicated by the nuclear influx of large cytoplasmic dextran polymers. Thus, kinesin-1 uncoats viral DNA and compromises NPC integrity, allowing viral genomes nuclear access to promote infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / physiology*
  • Adenoviridae Infections / metabolism*
  • Adenoviridae Infections / virology
  • Capsid / metabolism*
  • Cell Line
  • HeLa Cells
  • Humans
  • Kinesin / genetics
  • Kinesin / metabolism*
  • Nuclear Pore / genetics
  • Nuclear Pore / metabolism*
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / metabolism*
  • Protein Binding
  • Virus Assembly*

Substances

  • KIF5B protein, human
  • Nuclear Pore Complex Proteins
  • Kinesin