Secreted factors from Bifidobacterium animalis subsp. lactis inhibit NF-κB-mediated interleukin-8 gene expression in Caco-2 cells

Zhonggui Wang; Jinfeng Wang; Yi Cheng; Xin Liu; Ying Huang

doi:10.1128/AEM.06145-11

Secreted factors from Bifidobacterium animalis subsp. lactis inhibit NF-κB-mediated interleukin-8 gene expression in Caco-2 cells

Appl Environ Microbiol. 2011 Nov;77(22):8171-4. doi: 10.1128/AEM.06145-11. Epub 2011 Sep 16.

Authors

Zhonggui Wang¹, Jinfeng Wang, Yi Cheng, Xin Liu, Ying Huang

Affiliation

¹ Department of Biochemistry and Molecular Biology, The Second Clinical Hospital of Jilin University, Changchun 130041, People's Republic of China.

Abstract

The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflammatory factors might include a 50-kDa proteinaceous compound that is stable under a variety of heat and pH conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / isolation & purification
Anti-Inflammatory Agents / metabolism*
Bacterial Proteins / chemistry
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism
Bifidobacterium / metabolism*
Caco-2 Cells
Gene Expression / drug effects*
Humans
Hydrogen-Ion Concentration
Interleukin-8 / antagonists & inhibitors*
Molecular Weight
NF-kappa B / antagonists & inhibitors*
Protein Stability
Temperature

Substances

Anti-Inflammatory Agents
Bacterial Proteins
Interleukin-8
NF-kappa B