Production of endothelin-1 by rat cultured mesangial cells

Biochem Biophys Res Commun. 1990 Jun 15;169(2):462-8. doi: 10.1016/0006-291x(90)90354-p.


We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A) +RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Endothelins
  • Endothelium, Vascular
  • Glomerular Mesangium / metabolism*
  • Kinetics
  • Male
  • Peptide Biosynthesis*
  • Peptides / genetics
  • Peptides / isolation & purification
  • Protein Precursors / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Radioimmunoassay
  • Rats
  • Rats, Inbred Strains


  • Endothelins
  • Peptides
  • Protein Precursors
  • RNA, Messenger