Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 65 (9), 967-80

Review of Super-Resolution Fluorescence Microscopy for Biology


Review of Super-Resolution Fluorescence Microscopy for Biology

Bonnie O Leung et al. Appl Spectrosc.


Several methodologies have been developed over the past several years for super-resolution fluorescence microscopy including saturated structured-illumination microscopy (SSIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM), fluorescence photoactivation localization microscopy (FPALM), and stochastic optical reconstruction microscopy (STORM). While they have shown great promise for biological research, these techniques all have individual strengths and weaknesses. This review will describe the basic principles for achieving super resolution, demonstrate some applications in biology, and provide an overview of technical considerations for implementing these methods.

Similar articles

See all similar articles

Cited by 51 PubMed Central articles

See all "Cited by" articles

LinkOut - more resources