Prior endurance exercise prevents postprandial lipaemia-induced increases in reactive oxygen species in circulating CD31+ cells

J Physiol. 2011 Nov 15;589(Pt 22):5539-53. doi: 10.1113/jphysiol.2011.215277. Epub 2011 Sep 19.


We hypothesized that prior exercise would prevent postprandial lipaemia (PPL)-induced increases in intracellular reactive oxygen species (ROS) in three distinct circulating angiogenic cell (CAC) subpopulations. CD34(+), CD31(+)/CD14(-)/CD34(-), and CD31(+)/CD14(+)/CD34(-) CACs were isolated from blood samples obtained from 10 healthy men before and 4 h after ingesting a high fat meal with or without ∼50 min of prior endurance exercise. Significant PPL-induced increases in ROS production in both sets of CD31(+) cells were abolished by prior exercise. Experimental ex vivo inhibition of NADPH oxidase activity and mitochondrial ROS production indicated that mitochondria were the primary source of PPL-induced oxidative stress. The attenuated increases in ROS with prior exercise were associated with increased antioxidant gene expression in CD31(+)/CD14(-)/CD34(-) cells and reduced intracellular lipid uptake in CD31(+)/CD14(+)/CD34(-) cells. These findings were associated with systemic cardiovascular benefits of exercise, as serum triglyceride, oxidized low density lipoprotein-cholesterol, and plasma endothelial microparticle concentrations were lower in the prior exercise trial than the control trial. In conclusion, prior exercise completely prevents PPL-induced increases in ROS in CD31(+)/CD14(-)/CD34(-) and CD31(+)/CD14(+)/CD34(-) cells. The mechanisms underlying the effects of exercise on CAC function appear to vary among specific CAC types.

Publication types

  • Controlled Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Cell-Derived Microparticles
  • Diet, High-Fat*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Enzyme Inhibitors / pharmacology
  • Exercise*
  • Humans
  • Hyperlipidemias / metabolism*
  • Lipoproteins, LDL / blood
  • Male
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • NADPH Oxidases / antagonists & inhibitors
  • Nitric Oxide / metabolism
  • Oxidative Stress
  • Physical Endurance*
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism*
  • Postprandial Period / physiology
  • Reactive Oxygen Species / metabolism*
  • Triglycerides / blood
  • Young Adult


  • Enzyme Inhibitors
  • Lipoproteins, LDL
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Reactive Oxygen Species
  • Triglycerides
  • oxidized low density lipoprotein
  • Nitric Oxide
  • NADPH Oxidases