Atlas of Wnt and R-spondin gene expression in the developing male mouse lower urogenital tract

Dev Dyn. 2011 Nov;240(11):2548-60. doi: 10.1002/dvdy.22741. Epub 2011 Sep 20.


Prostate development is influenced by β-catenin signaling, but it is unclear which β-catenin activators are involved, where they are synthesized, and whether their mRNA abundance is influenced by androgens. We identified WNT/β-catenin-responsive β-galactosidase activity in the lower urogenital tract (LUT) of transgenic reporter mice, but β-galactosidase activity differed among the four mouse strains we examined. We used in situ hybridization to compare patterns of Wnts, r-spondins (Rspos, co-activators of β-catenin signaling), β-catenin-responsive mRNAs, and an androgen receptor-responsive mRNA in wild type fetal male, fetal female, and neonatal male LUT. Most Wnt and Rspo mRNAs were present in LUT during prostate development. Sexually dimorphic expression patterns were observed for WNT/β-catenin-responsive genes, and for Wnt2b, Wnt4, Wnt7a, Wnt9b, Wnt10b, Wnt11, Wnt16, and Rspo3 mRNAs. These results reveal sexual differences in WNT/β-catenin signaling in fetal LUT, supporting the idea that this pathway may be directly or indirectly responsive to androgens during prostate ductal development.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anatomy, Artistic
  • Animals
  • Animals, Newborn
  • Atlases as Topic
  • Female
  • Gene Expression Regulation, Developmental
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Thrombospondins / genetics*
  • Thrombospondins / metabolism
  • Tissue Distribution
  • Urogenital System / embryology*
  • Urogenital System / growth & development
  • Urogenital System / metabolism*
  • Wnt Proteins / genetics*
  • Wnt Proteins / metabolism
  • Wnt Signaling Pathway / genetics
  • Wnt Signaling Pathway / physiology


  • R-spondin3 protein, mouse
  • Thrombospondins
  • Wnt Proteins