Antioxidant and anti-inflammatory activities of Maillard reaction products isolated from sugar-amino acid model systems

J Agric Food Chem. 2011 Oct 26;59(20):11294-303. doi: 10.1021/jf2031583. Epub 2011 Oct 4.

Abstract

We investigated the antioxidant and anti-inflammatory activities of both crude and ultrafiltrated Maillard reaction (MR) products (MRPs) derived from sugar-amino acid MR models, comprising fructose (Fru), glucose (Glu) or ribose (Rib) reacted with glycine (Gly) or lysine (Lys), respectively. Crude MRPs derived from Glu-Lys showed the greatest capacity (P < 0.05) to inhibit nitric oxide (NO) and interleukin 8 (IL-8) production in interferon γ and phorbol ester-induced Caco-2 cells. Moreover, one ultrafiltrated fraction (MW < 1 kDa) recovered from Glu-Lys exhibited the greatest (P < 0.05) affinity to inhibit NO. This effect also corresponded to an inhibition of both iNOS transcription and translation. The NO and IL-8 inhibitory activities of crude MRPs were positively correlated with intracellular oxidation inhibitory activity. In conclusion, we have demonstrated an anti-inflammatory capacity of MRPs in inflamed Caco-2 cells that is specific to low molecular weight (MW < 1 kDa) Glu-Lys MRPs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry*
  • Anti-Inflammatory Agents / isolation & purification*
  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / isolation & purification*
  • Antioxidants / pharmacology
  • Caco-2 Cells
  • Carbohydrates / chemistry*
  • Fructose / chemistry
  • Glucose / chemistry
  • Glycine / chemistry
  • Humans
  • Interleukin-8 / antagonists & inhibitors
  • Lysine / chemistry
  • Maillard Reaction*
  • Nitric Oxide / antagonists & inhibitors
  • Ribose / chemistry

Substances

  • Amino Acids
  • Anti-Inflammatory Agents
  • Antioxidants
  • Carbohydrates
  • Interleukin-8
  • Fructose
  • Nitric Oxide
  • Ribose
  • Glucose
  • Lysine
  • Glycine