Analysis of single-nucleotide polymorphisms in patient-derived retrovirus integration sites reveals contamination from cell lines acutely infected by xenotropic murine leukemia virus-related virus

Alice Rusmevichientong; Jaydip Das Gupta; Petra S Elias; Robert H Silverman; Samson A Chow

doi:10.1128/JVI.05624-11

Analysis of single-nucleotide polymorphisms in patient-derived retrovirus integration sites reveals contamination from cell lines acutely infected by xenotropic murine leukemia virus-related virus

J Virol. 2011 Dec;85(23):12830-4. doi: 10.1128/JVI.05624-11. Epub 2011 Sep 21.

Authors

Alice Rusmevichientong¹, Jaydip Das Gupta, Petra S Elias, Robert H Silverman, Samson A Chow

Affiliation

¹ Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, UCLA School of Medicine, Los Angeles, CA 90095, USA.

Abstract

We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical to those in cell lines but not the patients, whereas the data on the remaining 12 integration sites were inconclusive. Our results provide direct evidence for contamination during analysis of XMRV integration sites.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Base Sequence
Humans
Male
Mice
Molecular Sequence Data
Polymorphism, Single Nucleotide / genetics*
Prostatic Neoplasms / genetics*
Prostatic Neoplasms / virology*
Proviruses / genetics
Retroviridae Infections / genetics
Retroviridae Infections / virology*
Sequence Homology, Nucleic Acid
Tumor Cells, Cultured
Virus Integration / genetics*
Xenotropic murine leukemia virus-related virus / genetics*
Xenotropic murine leukemia virus-related virus / isolation & purification
Xenotropic murine leukemia virus-related virus / pathogenicity