Branchiibius cervicis sp. nov., a novel species isolated from patients with atopic dermatitis

Abstract

Novel actinobacterial strains, PAGU 1247(T), PAGU 1251 and PAGU 1252, were isolated from the skin of atopic dermatitis patients and were characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were located within the family Dermacoccaceae. The most closely related species of PAGU 1247(T) in phylogenetic terms was Branchiibius hedensis Mer 29717(T), with 16S rRNA gene sequence similarity of 99.6%, although the DNA-DNA relatedness value was less than 43.9%. Some biochemical traits, such as lipase (C14) and α-galactosidase activity, could distinguish these isolates from B. hedensis. Strain PAGU 1247(T) contained iso-C(16:0) and brC(18:0) as the major fatty acids. The quinone system consisted of menaquinone MK-8(H(6) and H(4)). The G+C content of the genomic DNA was 67.6mol%. On the basis of its phenotypic properties and genetic distinctiveness, strains PAGU 1247(T), PAGU 1251 and PAGU 1252 represents a novel species of the genus Branchiibius, for which the name Branchiibius cervicis sp. nov. is proposed. The type strain is PAGU 1247(T) (=NBRC 106593(T)=DSM 24166(T)).