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. 1990 Mar;23(2):159-62.
doi: 10.1016/0147-619x(90)90036-c.

A yeast-Escherichia Coli Shuttle Vector Containing the M13 Origin of Replication

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A yeast-Escherichia Coli Shuttle Vector Containing the M13 Origin of Replication

N Rhodes et al. Plasmid. .

Abstract

A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.

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