Inhibitory Effects of Silver Nanoparticles on H1N1 Influenza A Virus in Vitro

J Virol Methods. 2011 Dec;178(1-2):137-42. doi: 10.1016/j.jviromet.2011.09.003. Epub 2011 Sep 17.

Abstract

Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • Apoptosis
  • Cell Line
  • Cell Survival
  • Chick Embryo
  • Dogs
  • Flow Cytometry
  • Hemagglutination Inhibition Tests
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Metal Nanoparticles*
  • Microbial Sensitivity Tests
  • Microscopy, Electron
  • Silver / pharmacology*
  • Survival Analysis

Substances

  • Antiviral Agents
  • Silver