c-Ki-ras mutations in chronic ulcerative colitis and sporadic colon carcinoma

Gastroenterology. 1990 Aug;99(2):416-20. doi: 10.1016/0016-5085(90)91024-z.


Mutations in the first exon of the c-Ki-ras protooncogene were analyzed in carcinomas and dysplasias from patients with sporadic colon cancer and chronic ulcerative colitis by a combination of histological enrichment, cell sorting, polymerase catalyzed chain reaction, and direct sequencing. In contrast to sporadic colon carcinomas, where 52% (11 of 21) contained mutations in codon 12, only 1 of 28 samples of ulcerative colitis associated carcinoma or dysplasia contained a c-Ki-ras mutation, despite the presence of aneuploid cell populations. These results suggest that a different genetic pathway for tumor progression may exist between sporadic colon carcinoma and carcinomas arising in chronic ulcerative colitis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Aneuploidy
  • Cell Separation
  • Colitis, Ulcerative / genetics*
  • Colonic Neoplasms / genetics*
  • Female
  • Flow Cytometry
  • Genes, ras*
  • Humans
  • Male
  • Middle Aged
  • Mutation*
  • Polymerase Chain Reaction
  • Proto-Oncogenes*