Abstract
We investigated the effect of topical epigallocatechin-3-gallate (EGCG) on testosterone (T)-induced hair loss in mice. Marked hair loss was observed at the T-injected site, and topical EGCG significantly reduced the hair loss (P < 0.05). TUNEL staining showed apoptosis of follicular epithelial cells in the T-injected groups where topical EGCG was found to significantly diminish T-induced apoptosis (P < 0.05). Topical EGCG down-regulated the T-induced expression of androgen receptor but did not down-regulate 17β-hydroxysteroid dehydrogenase (HSD) and three β-HSD expression. Analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) on serum and tissue samples revealed no significant difference in T and dihydrotestosterone concentrations between the T-injected and T + EGCG groups. Thus, we found that T injection in a mouse model induces hair loss by apoptosis of the hair follicles rather than through the androgen metabolic pathway and also saw that T-induced apoptosis of hair follicles was reduced by topical EGCG.
© 2011 John Wiley & Sons A/S.
MeSH terms
-
17-Hydroxysteroid Dehydrogenases / metabolism
-
3-Hydroxysteroid Dehydrogenases / metabolism
-
Alopecia / chemically induced
-
Alopecia / metabolism
-
Alopecia / pathology
-
Alopecia / prevention & control*
-
Animals
-
Antioxidants / administration & dosage
-
Antioxidants / pharmacology
-
Antioxidants / therapeutic use
-
Apoptosis / drug effects
-
Catechin / administration & dosage
-
Catechin / analogs & derivatives*
-
Catechin / pharmacology
-
Catechin / therapeutic use
-
Cyproterone Acetate / administration & dosage
-
Cyproterone Acetate / pharmacology
-
Cyproterone Acetate / therapeutic use
-
Dihydrotestosterone / metabolism
-
Disease Models, Animal*
-
Down-Regulation / drug effects
-
Epithelial Cells / cytology
-
Epithelial Cells / drug effects
-
Epithelial Cells / metabolism
-
Female
-
Hair / drug effects
-
Hair / metabolism
-
Hair Follicle / drug effects
-
Hair Follicle / metabolism
-
Hair Follicle / pathology
-
Mice
-
Mice, Inbred CBA
-
Receptors, Androgen / metabolism
-
Sebaceous Glands / drug effects
-
Sebaceous Glands / metabolism
-
Testosterone / metabolism
-
Testosterone / pharmacology*
Substances
-
Antioxidants
-
Receptors, Androgen
-
Dihydrotestosterone
-
Testosterone
-
Cyproterone Acetate
-
Catechin
-
epigallocatechin gallate
-
17-Hydroxysteroid Dehydrogenases
-
3-Hydroxysteroid Dehydrogenases