Hematopoietic stem cell development: using the zebrafish to identify the signaling networks and physical forces regulating hematopoiesis

Methods Cell Biol. 2011;105:117-36. doi: 10.1016/B978-0-12-381320-6.00005-9.

Abstract

Hematopoietic stem cells (HSC) form the basis of the hematopoietic hierarchy, giving rise to each of the blood lineages found throughout the lifetime of the organism. The genetic programs regulating HSC development are highly conserved between vertebrate species. The zebrafish has proven to be an excellent model for discovering and characterizing the signaling networks and physical forces regulating vertebrate hematopoietic development.

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Body Patterning / physiology
  • Cell Culture Techniques*
  • Cell Differentiation
  • Developmental Biology / methods*
  • Embryo, Nonmammalian / cytology*
  • Embryo, Nonmammalian / physiology
  • Flow Cytometry / methods*
  • Genes, Reporter
  • Hemangioblasts / cytology
  • Hematopoiesis / physiology*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / physiology
  • Hemodynamics
  • Mesoderm / cytology
  • Microscopy, Confocal / methods*
  • Mutation
  • Signal Transduction / physiology
  • Zebrafish / embryology
  • Zebrafish / physiology*
  • Zebrafish Proteins / analysis
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism

Substances

  • Biomarkers
  • Zebrafish Proteins