Nonmuscle myosin IIA is associated with poor prognosis of esophageal squamous cancer

Dis Esophagus. 2012 Jul;25(5):427-36. doi: 10.1111/j.1442-2050.2011.01261.x. Epub 2011 Sep 23.


Nonmuscle myosin IIA (myosin IIA) is a force-producing protein involved in the process of cell migration. Its expression has been considered as a bad prognostic indicator in stage I lung adenocarcinoma. However, the expression and clinical significance of myosin IIA in esophageal cancer has not been explored. In this study, we investigate the expression level of myosin IIA in 50 esophageal squamous cancer and 30 adjacent normal esophageal tissues by immunohistochemical staining and correlated its expression with clinicopathological features. Myosin IIA was expressed in all esophageal squamous cancer tissues (100%) and 8 of 30 adjacent normal tissues (26.7%, P = 0.000). In cancer tissues, elevated myosin IIA expression level was significantly correlated with increasing metastatic lymph nodes, poorer cancer differentiation, and advanced tumor stage. Further univariate analysis suggested that strong myosin IIA expression was associated with a significantly shorter overall survival (P = 0.021). In addition, MYH9 SiRNA was transfected into esophageal squamous cancer cell line (KYSE-510) to study the role of myosin IIA in cell migration. SiRNA-mediated depletion of myosin IIA in KYSE-510 cells significantly increased cell-matrix adhesion and attenuated cell migration ability (P = 0.000). In conclusion, these findings indicate that overexpression of myosin IIA may contribute to the progression and poor prognosis of esophageal squamous cancer, and this effect may be associated with increased cancer cell migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Cell Adhesion / physiology
  • Cell Line, Tumor
  • Cell Movement / physiology
  • Cell-Matrix Junctions / metabolism
  • Esophageal Neoplasms / genetics
  • Esophageal Neoplasms / metabolism*
  • Humans
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Molecular Motor Proteins / genetics
  • Molecular Motor Proteins / metabolism*
  • Myosin Heavy Chains / genetics
  • Myosin Heavy Chains / metabolism*
  • Nonmuscle Myosin Type IIA / genetics
  • Nonmuscle Myosin Type IIA / metabolism*
  • Nonmuscle Myosin Type IIA / physiology
  • Prognosis
  • RNA Interference / physiology
  • RNA, Small Interfering
  • Transfection


  • MYH9 protein, human
  • Molecular Motor Proteins
  • RNA, Small Interfering
  • Nonmuscle Myosin Type IIA
  • Myosin Heavy Chains