An alternative paper based tissue washing method for mass spectrometry imaging: localized washing and fragile tissue analysis

J Am Soc Mass Spectrom. 2011 Oct;22(10):1885-90. doi: 10.1007/s13361-011-0203-z. Epub 2011 Jul 19.


Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry
  • Female
  • Histocytological Preparation Techniques / methods*
  • Male
  • Mice
  • Molecular Imaging / methods*
  • Myocardium / chemistry
  • Paper
  • Peptides / chemistry
  • Proteins / chemistry
  • Rats
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Surface Properties


  • Peptides
  • Proteins