Biomimetic mineralization of dentin induced by agarose gel loaded with calcium phosphate

J Biomed Mater Res B Appl Biomater. 2012 Jan;100(1):138-44. doi: 10.1002/jbm.b.31931. Epub 2011 Sep 27.


A novel biomimetic mineralization system was designed to induce a layer of hydroxyapatite on a demineralized dentin surface. This system was constructed as follows. A layer of 0.5% agarose gel containing 0.26M Na(2) HPO(4) was used to cover acid-etched dentin slices, followed by a layer of agarose gel without phosphate ions. Then a neutral 0.13M CaCl(2) solution was added onto the ion-free gel surface. The mineralization system (dentin-agarose gel containing phosphate ions-CaCl(2) solution) was kept in a water bath at 37°C, and the gel and CaCl(2) solution were replaced at various intervals. The results showed that the deposited hydroxyapatite crystals densely packed to each other, completely covered the dentin surface, and occluded the dentinal tubules after 10 days of biomimetic mineralization in vitro. Therefore, this method may provide the experimental basis for dentin remineralization and for a new method to treat dentin hypersensitivity and dental caries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomimetic Materials / chemical synthesis*
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / therapeutic use
  • Dental Caries / therapy
  • Dentin / chemistry*
  • Dentin Sensitivity / therapy
  • Durapatite / chemical synthesis*
  • Durapatite / chemistry
  • Durapatite / therapeutic use
  • Gels / chemistry
  • Humans
  • Sepharose / chemistry*


  • Gels
  • Sepharose
  • Durapatite