High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

Analyst. 2011 Nov 21;136(22):4670-3. doi: 10.1039/c1an15684e. Epub 2011 Sep 29.


A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Glycomics / methods*
  • Humans
  • Polysaccharides / blood*
  • Spectrometry, Fluorescence / methods*
  • Staining and Labeling / methods*


  • Polysaccharides