Pre-mRNA 3'-end processing complex assembly and function

Wiley Interdiscip Rev RNA. May-Jun 2011;2(3):321-35. doi: 10.1002/wrna.54. Epub 2010 Oct 18.

Abstract

The 3'-ends of almost all eukaryotic mRNAs are formed in a two-step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly-adenosine or poly(A) tail). These reactions take place in the pre-mRNA 3' processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre-mRNA 3' processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges.

Publication types

  • Review

MeSH terms

  • Animals
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / metabolism
  • Humans
  • Macromolecular Substances / chemistry
  • Macromolecular Substances / metabolism
  • Models, Biological
  • Plants / genetics
  • Plants / metabolism
  • Poly(A)-Binding Proteins / chemistry
  • Poly(A)-Binding Proteins / metabolism
  • Polynucleotide Adenylyltransferase / chemistry
  • Polynucleotide Adenylyltransferase / metabolism
  • Protein Interaction Domains and Motifs
  • RNA 3' End Processing / physiology*
  • RNA 3' Polyadenylation Signals
  • RNA Precursors / chemistry
  • RNA Precursors / genetics
  • RNA Precursors / metabolism*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • mRNA Cleavage and Polyadenylation Factors / chemistry
  • mRNA Cleavage and Polyadenylation Factors / metabolism

Substances

  • Macromolecular Substances
  • Poly(A)-Binding Proteins
  • RNA Precursors
  • RNA-Binding Proteins
  • mRNA Cleavage and Polyadenylation Factors
  • Polynucleotide Adenylyltransferase
  • DNA-Directed RNA Polymerases