Establishment of new human prostatic cancer cell line (JCA-1)

Urology. 1990 Jul;36(1):79-84. doi: 10.1016/0090-4295(90)80319-i.

Abstract

The establishment of a new human prostatic cancer cell line is described. This cell line was derived from a poorly to moderately differentiated prostatic adenocarcinoma. It has been maintained in tissue culture for fourteen months and has been passed fifty-two times. This cell line has an ability to form colonies in soft agar suspension cultures, and also is transplantable to nude mice. Tumors grown in nude mice revealed a poorly differentiated adenocarcinoma with positive PSA staining. Acid phosphatase activity was detected in freeze-thawed cells by enzymatic assay. A karyotype analysis demonstrated aneuploidy with a model chromosomal number of 69 and six marker chromosomes.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism
  • Adenocarcinoma / enzymology
  • Adenocarcinoma / genetics
  • Adenocarcinoma / pathology*
  • Aged
  • Aneuploidy
  • Animals
  • Biomarkers, Tumor / analysis
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Karyotyping
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / pathology*
  • Tumor Cells, Cultured*

Substances

  • Biomarkers, Tumor
  • Acid Phosphatase